Plasmid

Part:BBa_K3776020:Design

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)


panS-SPIN-davBA-AK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7311
    Illegal NheI site found at 9398
    Illegal NheI site found at 13516
    Illegal NotI site found at 33
    Illegal NotI site found at 15236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
    Illegal BglII site found at 6737
    Illegal BglII site found at 7528
    Illegal BglII site found at 8058
    Illegal BglII site found at 13303
    Illegal BglII site found at 16325
    Illegal BglII site found at 19280
    Illegal BamHI site found at 6
    Illegal BamHI site found at 5880
    Illegal BamHI site found at 14431
    Illegal XhoI site found at 5663
    Illegal XhoI site found at 14414
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2378
    Illegal NgoMIV site found at 16485
    Illegal NgoMIV site found at 18252
    Illegal AgeI site found at 4103
    Illegal AgeI site found at 13435
    Illegal AgeI site found at 15892
    Illegal AgeI site found at 16141
    Illegal AgeI site found at 17476
    Illegal AgeI site found at 19479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5846
    Illegal BsaI.rc site found at 5820
    Illegal SapI.rc site found at 8814


Design Notes

Integrate system with pANS for S.elongatus UTEX 2973


Source

Integrate system with pANS for S.elongatus UTEX 2973

References